146. MECHANICAL PROPERTIES OF VASCULAR ENDOTHELIAL CELLS EXPOSED TO STRETCH

Department: Mechanical & Aerospace Engineering
Faculty Advisor(s): Juan Carlos del Alamo
Award(s): Department Best Poster

Primary Student
Name: Kathryn Elizabeth Osterday
Email: kosterda@ucsd.edu
Phone: 530-417-4711
Grad Year: 2015

Student Collaborators
Thomas Chew, thomas.chew@gmail.com | Phillip Loury, pjloury@gmail.com | Manuel Gomez-Gonzalez, mgomezgo@ucsd.edu

Abstract
The pressure pulse present in arteries exposes vascular endothelial cells (VECs) to stretch. It is known that, under cyclic, uniaxial stretch, VECs align their actin filaments perpendicular to the direction of stretch. We study how this cytoskeletal remodeling is correlated to changes in subcellular microrheology. We analyzed the changes in the magnitude and directionality of the shear and elastic moduli of bovine aortic endothelial cells (BAECs) exposed to cyclical, uniaxial stretch using Directional Particle Tracking Microrheology (DPTM) that employs novel microrheology formulea that better account for the anisotropy of the cytoplasm. We find that, under cyclic, uniaxial stretch, BAECs stiffen and align their softest direction of mechanical polarization perpendicular to stretch. We hypothesize that the response of VECs to stretch acts to minimize intracellular strain in response to stress.

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