16. ISOLATION AND ON-CHIP PCR AMPLIFICATION OF DISEASE RELATED DNA NANOPARTICULATE BIOMARKERS: SEAMLESS SAMPLE-TO-ANSWER INTEGRATED DIAGNOSTICS

Department: Bioengineering
Faculty Advisor(s): Michael Heller

Primary Student
Name: Avery R Sonnenberg
Email: asonnenb@ucsd.edu
Phone: 716-485-1412
Grad Year: 2013

Abstract
Cell-free circulating (CFC) DNA/RNA and other cellular nanoparticulates are being increasingly studied for their potential as biomarkers for early detection of cancer and other diseases. In prior work, we developed a novel dielectrophoretic (DEP) method that allows the isolation and detection of DNA nanoparticulates directly from whole blood on a microarray device. We demonstrated the separation of high molecular weight (HMW) DNA and fluorescent particles in the 40 nm to 200 nm range from whole blood. Using the same microelectrode array and technique, we observed visible quantities of SYBR Green fluorescent-stained DNA in Chronic Lymphocytic Leukemia (CLL) patient blood samples. New microarray devices have now been constructed that increase the ability to collect nanoparticulate material as well as allow the integration of the temperature control necessary to perform PCR thermal cycles. With the sample collection and analysis conducted in the same fluidic device, we have eliminated the need for any off-chip processing and created a truly seamless "sample-to-answer" diagnostic device for CFC-DNA based early disease diagnostics.

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