11. DYNAMIC ENVIRONMENT MICROCHEMOSTAT FOR EVOLUTIONARY EXPERIMENTS

Department: Bioengineering
Faculty Advisor(s): Jeff Hasty

Primary Student
Name: Ivan Alexandrovich Razinkov
Email: irazinko@ucsd.edu
Phone: 858-822-2223
Grad Year: 2012

Abstract
Evolution of new phenotype and genotypes has been done using various methods: batch culture, plates, liquid culture and chemostats. By using chemostats it is possible to precisely control and monitor the cell's environment, while maintaining the culture for extended periods of time, from months to years. However, maintaining the chemostats requires large amounts of media and supplies, which in some cases can be quite expensive. In addition, due to the inherent design of chemostats with cells suspended in the media, it is impossible to achieve dynamic activation or inhibition of cell processes. In this work we design a microfluidic chemostat, microchemostat, that is able to culture a large number of cells, while maintaining the ability for dynamic switching of the environment. This technology can be used for long term cell monitoring or evolution of specific cell strains, while consuming minimal amounts of reagents.

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