134. FABRICATION OF MONO-SIZED MESOPORES ON GOLD-COATED POLYSTYRENE PARTICLE SURFACES FOR ENZYME IMMOBILIZATION

Department: Mechanical & Aerospace Engineering
Research Institute Affiliation: Graduate Program in Materials Science and Engineering
Faculty Advisor(s): Olivia A. Graeve

Primary Student
Name: Seongcheol Choi
Email: sec047@ucsd.edu
Phone: 858-534-8262
Grad Year: 2018

Abstract
Mono-sized mesoporous gold-coated PS (polystyrene) particles, which are characterized by their controllable pore size, were prepared to investigate their use as scaffolds for support of CPO (chloroperoxidase) enzymes for the purpose of maximization of the activity and stability of their enzymatic activity. First, mono-sized polystyrene particles were fabricated in water by soap-free emulsion polymerization with a thermal initiator. Second, the PS particles were functionalized by covering their surface with PAA (polyallylamine) to convert their negative charge to positive in water. Third, mono-sized silica nanoparticles were synthesized by using a sol-gel method in water with L-lysine, which acts as a dispersing agent and a basic catalyst. Fourth, this mono-sized silica nanospheres were electrostatically deposited in isolation on the PAA-functionalized PS particles by a pH adjustment. Finally, ultra-thin gold layers were coated not on the surface of silica nanoparticles but on primary amine group exposed surface of PAA on PS particles. Subsequently, the silica nanoparticles were etched with sodium hydroxide solution at 70 to 80C. The morphology of the mesoporous particles will be described and their properties as promising enzyme substrates will be analyzed.

Industry Application Area(s)
Energy/Clean technology | Life Sciences/Medical Devices & Instruments | Materials

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