176. a novel dielectrophoresis platform for the isolation of extracellular vesicles

Department: NanoEngineering
Faculty Advisor(s): Sadik C. Esener

Primary Student
Name: Lennart Langouche
Email: llangouc@ucsd.edu
Phone: 858-291-2562
Grad Year: 2019

Abstract
Cells release into the extracellular environment diverse types of membrane-bound vesicles such as exosomes and microvesicles. These extracellular vesicles (EVs) contain proteins, lipids, and nucleotides, mediating intercellular communication between different cell types in the body, and thus affecting normal and pathological conditions. Research in this field has grown rapidly, and there is a wide range of techniques available for the isolation and characterization of EVs. A lot of these techniques are poorly standardized, which makes comparison of data from different studies difficult. We present a new platform that enables a purely physical method of isolation, using dielectrophoresis (DEP). This platform uses electrical fields to separate EVs from other sample components based on inherent differences in dielectric properties. This technology could allow for a standardized isolation method that is faster than current technologies, concentrates larger amounts of EVs, and reduces sample contamination. Applications are not limited to EVs only as our platform could also be used for the recovery of other biomarkers or drug delivery vehicles directly from sample.

Industry Application Area(s)
Electronics/Photonics | Life Sciences/Medical Devices & Instruments

Related Links:

  1. https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-016-0268-z

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