177. rapid sample to answer diagnostics for traumatic brain injury

Department: NanoEngineering
Research Institute Affiliation: Graduate Program in Chemical Engineering
Faculty Advisor(s):

Primary Student
Name: Benjamin Gabriel Sarno
Email: bsarno@ucsd.edu
Phone: 858-534-9897
Grad Year: 2017

Abstract
Rapid Sample to Answer Diagnostics for Traumatic Brain Injury Annually, 1.7 million Americans suffer a traumatic brain injury (TBI). Of these patients, 52,000 die, 275,000 face serious hospitalization and medical care, while another 1.365 million experience emergency treatment and are discharged. Quick and accurate diagnosis of TBI severity is a key component in determining patient care and processing. Unfortunately, time consuming sample preparation and processing leads physicians to more uncertain, judgement based diagnosis. A more rapid, non-invasive diagnostic method with unique advantages may now be available by using AC dielectrophoretic (DEP) microarray technology. We investigated TBI and brain cancer plasma samples versus healthy subject samples, and discovered connections between exosome biomarkers from brain cancer and TBI. We utilized a DEP electrode array arranged on a microchip device to rapidly isolate brain cancer and TBI exosomes from 25ÁL of plasma. Immunofluorescence was used to identify exosome related protein biomarkers markers, such as CD63 and glypican-1, directly on-chip. Sample processing plus exosome isolation took less than 25 minutes. This DEP microchip isolation technology has the ability to advance rapid and effective TBI detection and diagnosis and lead to a point-of- care molecular diagnostic.

Industry Application Area(s)
Aerospace, Defense, Security | Electronics/Photonics | Life Sciences/Medical Devices & Instruments

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