184. characterization of bsa adsorption on heterogeneously pegylated ag nps
Name: Madhura Som
Grad Year: 2019
PEGylation is a widely used strategy to engineer nanoparticle surfaces to be stable and resistant to non-specific protein adsorption in a blood-like biological environment. Non-specific protein adsorption in in-vivo environments usually means that nanomaterials lose their colloidal stability and get tagged by the immune system for rapid clearance from circulation. PEGylation cannot completely prevent protein adsorption since small proteins and organothiols can still pass through its void spaces and get adsorbed directly on the nanoparticle surfaces. This work explores a heterogenous Ag NP surface chemistry consisting of small alkanethiols passivating the NP surface in addition to PEG in a mixed self-assembled monolayer. We use a representative serum protein, i.e. Bovine Serum Albumin (BSA) in an in-vitro buffered environment to monitor the kinetics of BSA adsorption on such a heterogeneous surface. Our studies confirm that the mixed PEGylated Ag NP surface is more efficacious in preventing irreversible protein corona formation over extended periods of time in a protein containing bio-environment.
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