53. probing mechanobiology with laser-induced shockwaves

Department: Bioengineering
Faculty Advisor(s): Michael Berns

Primary Student
Name: Christopher Carmona
Email: c2carmon@ucsd.edu
Phone: 858-534-4194
Grad Year: 2020

Student Collaborators
Daryl Preece, dpreece@ucsd.edu | Linda Shi, zhixiashi@gmail.com | Veronica Gomez-Godinez, gomezvg@gmail.com

Traumatic Brain Injury (TBI) occurs when an external force injures the brain. According to the Center of Disease Control and Prevention, TBI is a contributing factor to a third of all injury-related deaths in the United States. Those that survive TBI can face temporary or permanent effects that significantly impact thinking or memory, movement, sensation, or emotional functioning. While clinical outcomes of TBI can vary widely in severity, few mechanisms of neurodegeneration following TBI have been identified for treatment. We propose a model for studying TBI using laser-induced shockwaves (LISs). To further our understanding on the way that cells respond to shear stress, an optical system was developed that allows single cells to be studied in response to a sudden but short application of shear stress. This system can generate LISs at a specified time and location. The LIS exerts a shear stress between 0 - 50 kPa depending on the distance from the shockwave epicenter. Our system utilizes an optically-coupled force measurement component that allows for the visualization of shockwave dynamics. Here, the force measurement system is characterized by imaging stages of the approximately 40 ns period of violent expansion and collapse of microbubbles responsible for shockwave generation. Ultimately, this system?s capacity to quantitatively study shockwave dynamics permits the study of the spatiotemporal details occurring in cells in response to shear stress. Thus, TBI can be further studied and subsequent results could reveal the molecular basis for neurodegeneration and potential therapeutic avenues for treatment.

Industry Application Area(s)
Electronics/Photonics | Life Sciences/Medical Devices & Instruments

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