63. extended photonic transfer in dna nanostructures

Department: Bioengineering
Research Institute Affiliation: California Institute for Telecommunications and Information Technology (Calit2)
Faculty Advisor(s):

Primary Student
Name: Alaleh Golkar Narenji
Email: agolkarn@ucsd.edu
Phone: 858-534-9897
Grad Year: 2017

Abstract
DNA demonstrates a remarkable capacity for creating nanostructures and devices that can potentially be used in biocomputing, sensing, electronic, biosynthetic, drug delivery and plasmonic devices. A growing number of these structures utilize fluorescence resonance energy transfer (FRET) as part of the device?s functionality, readout and characterization. A particularly useful property to impart into DNA constructs would be long range or extended FRET. Our group has now shown that that the use of surfactants and metal cations results in long range FRET enhancement in DNA structures with multiple fluorescent dyes. Combinations of multiple donor florescence dyes with a single acceptor dye were investigated. Increasing the number of these dyes attached to the DNA construct results in FRET efficiency improvement. Moreover, the positive cations and positive polarity surfactants appear to neutralize the DNA backbone resulting in reducing the dye quenching and dimerization. To examine the mechanism in detail, DNA structures were investigated by fluorescence emission spectroscopy and dynamic light scattering (DLS).

Industry Application Area(s)
Electronics/Photonics | Life Sciences/Medical Devices & Instruments | Materials

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