64. rapid electrophoretic method for the detection of enzyme activities in unprocessed blood

Department: Bioengineering
Faculty Advisor(s):

Primary Student
Name: Elaine Alexandra Skowronski
Email: eskowron@ucsd.edu
Phone: 760-717-9237
Grad Year: 2017

Abstract
Enzymatic activity of lipases, amylases, and nucleases have been linked to a variety of diseases and monitoring of abnormal up or down regulation of this activity could provide actionable information to doctors for patient management decisions. A rapid electrophoretic method has been developed to detect enzymatic activities of lipases, amylases, and nucleases in unprocessed blood. Substrates are designed to be cleaved by specific enzymes and to change charge from either net neutral or negative to positive upon cleavage. After incubation of fluorescently labeled substrates with microliters of unprocessed patient blood, the substrates are cleaved by the for which they have been designed and yield positively charged and fluorescently labeled cleavage products. Upon application of an electric field the positively charged and fluorescently labeled cleavage products are rapidly separated from blood, which is composed mostly of negatively charged components. Quantification of the fluorescent signal indicates the activity of specific enzymes in the circulation of the patient.

Industry Application Area(s)
Life Sciences/Medical Devices & Instruments

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