35. comparison of deproteinization methods for porcine femur cortical bone

Department: Mechanical & Aerospace Engineering
Research Institute Affiliation: Agile - CaliBaja Center for Resilient Materials & Systems
Faculty Advisor(s): Joanna M. McKittrick

Primary Student
Name: Frances Yenan Su
Email: fysu@ucsd.edu
Phone: 626-802-7389
Grad Year: 2018

Scientists and engineers have long struggled to mimic the shape and size of biogenic hydroxyapatite for use in osteoconductive bone implants. While synthetic hydroxyapatite particles are round, biogenic hydroxyapatite are thin platelets. Instead of synthesizing platelet hydroxyapatite, deproteinization, or removing protein from bone, is a different method of producing biogenic hydroxyapatite. Previous studies have shown that 2.6 wt.% sodium hypochlorite, 7 wt.% hydrogen peroxide, 1N potassium hydroxide, and 1N sodium hydroxide are effective in deproteinizing bone. Since bone also contains fats, a defatting step using a 2:1 chloroform-methanol solution was added to the sodium hypochlorite treatment. Results of treatments were analyzed using x-ray diffraction to verify that the crystal structure of hydroxyapatite was not affected by the treatments. Thermogravimetric analysis and Raman spectroscopy were used to determine if there was remaining protein in the samples. This research is supported by the National Science Foundation (DMR-1507978).

Industry Application Area(s)
Civil/Structural Engineering | Life Sciences/Medical Devices & Instruments | Materials

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