microfluidic development for the simultaneous culturing of 2,048 unique e. coli and s. cerevisiae strains

Department: Bioengineering
Faculty Advisor(s): Jeff M. Hasty

Primary Student
Name: Nicholas Sigmund Csicsery
Email: ncsicser@ucsd.edu
Phone: 858-534-9899
Grad Year: 2018

Student Collaborators
Elizabeth Stasiowski, estasiow@ucsd.edu | Garrett Graham, ggraham@eng.ucsd.edu | Gregoire Thouvenin, gthouven@eng.ucsd.edu

Microfluidic technology enables the imaging of microbial colonies under continuous growth conditions with precise environmental control. Applications such as environmental biosensors or genome-wide expression analysis require large-scale microfluidic devices capable of culturing and monitoring thousands of microbial strains exposed to a uniform environment. Here we have developed a microfluidic device capable of simultaneously culturing 2,048 unique strains of E. coli or S. cerevisiae. Trap geometries designed for optimal cell retention and fluorescent signal enable gene expression at the colony level to be measured by custom in-house optics. As an initial application, we have constructed an in-situ biosensor which can monitor thousands of sensor strains in real-time. With this technology, we have screened fluorescent libraries totaling over 6,000 E. coli and S. cerevisiae strains against multiple environmental toxins, identifying a number of naturally responsive genes in these organisms to be used in a final biosensor device.

Industry Application Area(s)
Life Sciences/Medical Devices & Instruments

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