microfluidic platform for neutrophil-like hl60 transendothelial migration in static conditions

Department: Mechanical & Aerospace Engineering
Faculty Advisor(s): Juan C. Lasheras

Primary Student
Name: Amy Beth Schwartz
Email: abschwar@ucsd.edu
Phone: 858-534-4368
Grad Year: 2021

The transendothelial migration (TEM) of neutrophils, the most common type of white blood cell, is an essential physiological process that regulates neutrophil response to cellular distress signals issued at sites of infection as part of the inflammatory response. In a well-documented process, passing neutrophils are captured by and adhere to activated endothelial cells lining the blood vessel walls, undergo extravasation through the endothelial layer, and migrate toward the site of inflammation. In a preliminary step toward decoupling the mechanical contributions of neutrophils and endothelial cells in the TEM process, this study presents a methodology for the fabrication and application of a microfluidic platform to mimic the in vivo neutrophil TEM process. A collagen hydrogel scaffold supporting a monolayer of Human Umbilical Vein Endothelial Cells (HUVECs) is embedded in the center of the device, with side channels allowing for the development of a highly stable chemoattractant gradient to induce neutrophil-like differentiated Human promyelocytic leukemia cells (HL60) extravasation across the monolayer and into the collagen scaffold. Cytoplasmic and mitochondrial staining help to provide an early assessment of the correlation between collagen curvature and HUVEC adhesion, in addition to evidence of clear and rapid chemotaxis of HL60 cells in a static fluid environment.

Industry Application Area(s)
Life Sciences/Medical Devices & Instruments

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