55. characterizing forces imparted on cells using laser-induced shockwaves

Department: Bioengineering
Faculty Advisor(s): Michael Berns

Primary Student
Name: Christopher Carmona
Email: c2carmon@ucsd.edu
Phone: 213-700-6729
Grad Year: 2021

Abstract
Traumatic Brain Injury (TBI) occurs when an external force injures the brain. While clinical outcomes of TBI can vary widely in severity, few mechanisms of neurodegeneration following traumatic brain injury (TBI) have been identified for treatment. Understanding mechanotransduction in cells is key to understanding cellular response to injury. We propose a model utilizing photodisruption for studying the early pathogenesis of TBI in neurons by generating laser-induced shockwaves (LISs). Photodisruption allows for the generation of spatiotemporally defined shear stress against cells. An optical system was developed that allows single cells to be selectively studied in response to LISs. The force measurement system is characterized by imaging stages over the period of cavitation then, violent expansion and collapse of microbubbles responsible for shockwave generation.

Industry Application Area(s)
Electronics/Photonics | Life Sciences/Medical Devices & Instruments

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